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free iso.moved.to.zenshare.org/w/j/f0/e75993649de1168c543bbc14c2a9a6e8$59.49.95.95Improving the thermodynamic stability of an enzymatic catalyst by site-directed mutagenesis.
A series of mutants of a thermostable triosephosphate isomerase (TIM) with improved stability were designed and characterized. Site-directed mutagenesis was employed to introduce charged residues in surface areas that may influence the stability of the protein. These mutants displayed significant increases in stability. The resistance to thermal denaturation was monitored by differential scanning calorimetry (DSC) and the unfolding equilibrium constant for folding was determined as a measure of the thermal stability of each mutant. The T61A, T63A, D64A, and E65A mutants displayed up to a 70-fold increase in thermal stability as compared to wild-type TIM. These results indicate that an increase in the number of charged residues in the surface of TIM can improve the stability of the enzyme, presumably by decreasing the exposure of hydrophobic regions of the protein to water. However, increased thermal stability also increased the catalytic activity of TIM. Although these mutants were thermodynamically stable, they had an altered catalytic mechanism.I am a bit confused about the nomenclature of astrocytes and their roles in disease. «Classic» astrocyte marker(s) such as GFAP, S100β, vimentin have been established and have contributed to a better understanding of astrocyte phenotypes. In contrast, the field has recently turned to secreted proteins as useful markers of astrocyte populations. For example, S100β, a calcium-binding protein is expressed by both classic and non-classic astrocytes and is useful as an astrocyte marker. It is more specific than GFAP for distinguishing astrocytes in high-density cultures, but is expressed in low numbers in adult brain. S100β is especially useful for identifying astrocytes in acute brain slices where it is readily detectable with simple immunostaining procedures. However, this marker does not always reveal differences in astrocyte populations, and it is not expressed by reactive astrocytes, and its expression persists in mature astrocytes (

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OGM JAF PKEY Emulator V5.rar. OGM JAF PKEY Emulator v5.exe is an ultimate key & license key simulator for real-time work. In place of license key,.Q:

Add custom fields to nodes form submission

I’m looking for a module that adds custom fields to the node submission form.
For instance, say I wanted to add an extra field that is hidden and just stores a short message. It needs to be available to the end user, but doesn’t need to be exposed on the form, so it doesn’t need to be an exposed field.
Is this possible?

A:

I think this is what you want.

Q:

Not the clear case of ‘this’ in Javascript

Today I come across with the syntax of this. The code are like below:
function x(){
var that = this;
this.c = function(){
alert(that);//It is error!!
}
}
x.prototype.a = 1;
var a = new x();

I have thought that that’s to mimic the properties of object, and the value of that will be the same as this so that’s why the error also exist. But the result of ‘that’ in the code above is undefined.
So, anyone could explain to me about this concept in javascript?

A:

When you do
function x() {
this.c = function() {
alert(this); // error!
}
}
x.prototype.a = 1;
var a = new x();

this is evaluated and hence, the value of that in the inner function is the object of the instance of the constructor function x.
If you had written:
function x() {
var that = this;
this.c = function() {
alert(that);

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