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*GFP*-*vimentin*, *GFP*-*α*-*actinin* and *GFP*-*slam* constructs were generated in pCS2+ and transfected into *X. laevis* embryos. Two and a half days after injection, embryos were removed from the chorion and dechorionated in 0.8% (w/v) cysteine HCl (Sigma-Aldrich, Gillingham, UK) in nuclease-free (NF) water for one hour at room temperature. After two washes in NF water, embryos were homogenised in lysis buffer (30 mM Tris-HCl, 5 mM MgCl~2~, 0.2% (v/v) Triton X-100, 0.5 mM PMSF) supplemented with protease inhibitor cocktails (Sigma) on ice and then centrifuged for 10 minutes at 15,000 × *g* at 4 °C. The supernatant was
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